## ----setup, include = FALSE---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------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knitr::opts_chunk$set(
    collapse = TRUE,
    comment = "#>",
    crop = NULL ## Related to https://stat.ethz.ch/pipermail/bioc-devel/2020-April/016656.html
)
options(width = 9999)

## ----vignetteSetup, echo=FALSE, message=FALSE, warning = FALSE-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------
## Bib setup
library("RefManageR")
bib <- c(
    R = citation(),
    InteractionSet = citation("InteractionSet")[1],
    BiocStyle = citation("BiocStyle")[1],
    knitr = citation("knitr")[1],
    RefManageR = citation("RefManageR")[1],
    rmarkdown = citation("rmarkdown")[1],
    sessioninfo = citation("sessioninfo")[1],
    testthat = citation("testthat")[1],
    dplyr = citation("dplyr")[1],
    rlang = citation("rlang")[1],
    plyranges = citation("plyranges")[1],
    plyinteractions = citation("plyinteractions")[1]
)

## 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library(GenomicRanges)
library(InteractionSet)
anchor1 <- GRanges("chr1:10-20:+")
anchor2 <- GRanges("chr1:50-60:-")
gi <- GInteractions(anchor1, anchor2)

gi

## ----"start", message=FALSE-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------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## This uses an example `bedpe` file provided in the `rtracklayer` package
bedpe_file <- system.file("tests", "test.bedpe", package = "rtracklayer")
bedpe_df <- read.delim(bedpe_file, header = FALSE, sep = '\t')

bedpe_df

## 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library(plyinteractions)
gi <- bedpe_df |> 
    as_ginteractions(
        seqnames1 = V1, start1 = V2, end1 = V3, strand1 = V9, 
        seqnames2 = V4, start2 = V5, end2 = V6, strand2 = V10, 
        starts.in.df.are.0based = TRUE
    )

gi

## 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## This uses an example `pairs` file provided in this package
pairs_file <- system.file('extdata', 'pairs.gz', package = 'plyinteractions') 
pairs_df <- read.delim(pairs_file, sep = "\t", header = FALSE, comment.char = "#")
head(pairs_df)
pairs <- as_ginteractions(pairs_df, 
    seqnames1 = V2, start1 = V3, strand1 = V6, 
    seqnames2 = V4, start2 = V5, strand2 = V7, 
    width1 = 1, width2 = 1, 
    keep.extra.columns = FALSE
)
pairs

## 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tibble::as_tibble(gi)

## 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gi <- read.table(text = "
chr1 1 10 chr1 1 15 + + cis
chr1 6 15 chr1 1 20 + + cis
chr1 6 20 chr1 6 30 - - cis
chr1 11 30 chr2 11 30 - - trans",
col.names = c(
  "seqnames1", "start1", "end1", 
  "seqnames2", "start2", "end2", "strand1", "strand2", 
  "type")
) |> 
  as_ginteractions()

## `anchors` returns the two sets of anchors (i.e. "left" and "right" 
## loci) for each genomic interaction

anchors(gi)

## `anchors1` and `anchors2` specifically return the "left" OR "right" 
## loci) for each genomic interaction

anchors1(gi)

anchors2(gi)

## 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## Similarly to `GRanges` accessors, `seqnames`, `start`, `end`, `strand` and 
## `width` are all available for each set of `anchors` of a `GInteractions`. 

seqnames1(gi)

start1(gi)

end2(gi)

strand2(gi)

width2(gi)

## 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mcols(gi)

## 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gi$type

## 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regions(gi)

seqinfo(gi)

## 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## `pin_by` is used to pin a `GInteractions` on "first" (i.e. "left") or 
## "second" (i.e. "right") anchors. 

gi |> pin_by("first")

pgi <- gi |> pin_by("second")
pin(pgi)

pinned_anchors(pgi)

## 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unpin(pgi)

## 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gi |> pin_by("first") |> anchor_5p()

## 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gi

## This pins the "first" (i.e. "left") anchors and strecthes them by 10bp

gi |> pin_by("first") |> stretch(10)

## This pins the "first" (i.e. "left") anchors and shift them 
## by 20bp to the right

gi |> pin_by("first") |> shift_right(20)

## This pins the "first" (i.e. "left") anchors and extracts 20bp 
## flanking these "first" anchors

gi |> pin_by("first") |> flank_right(20)

## ----pin_anchor_stretch----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------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## This pins the "first" (i.e. "left") anchors and strecthes them by 10bp, 
## with the "first" anchors being anchored at their **start**. 

gi |> pin_by("first") |> anchor_start() |> stretch(10)

## This pins the "first" (i.e. "left") anchors and strecthes them by 10bp, 
## with the "first" anchors being anchored at their **center**. 

gi |> pin_by("first") |> anchor_center() |> stretch(10)

## 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## This creates a new metadata column named `cis`

gi |> mutate(cis = seqnames1 == seqnames2)

## This creates a new metadata column named `both_chr`

gi |> mutate(both_chr = paste(seqnames1, seqnames2, sep = "_"))

## This modifies `start1`, i.e. the `start` coordinates of the "first"
## (i.e. "left") anchors of the `GInteractions` object. 

gi |> mutate(start1 = 1)

## 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## This groups the `GInteractions` object using the `seqnames2` variable
## (i.e. the `seqnames` of the "second" anchors of the `GInteractions`). 

gi |> group_by(seqnames2)

## This groups the `GInteractions` object by a new variable named `cis`

gi |> group_by(cis = seqnames1 == seqnames2)

## This groups the `GInteractions` object by two variables, `seqnames2` 
## and the new variable `cis`

gi |> group_by(seqnames2, cis = seqnames1 == seqnames2)

## 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## This counts the number of occurences of each combination of the variables
## `strand1` and `strand2`

pairs |> count(strand1, strand2)

## This counts the number of pairs located on the same strand 
## or different strands

gi |> group_by(same_strand = strand1 == strand2) |> tally()

## This counts the number of pairs located on the same strand 
## or different strands

pairs |> group_by(same_strand = strand1 == strand2) |> 
    summarize(
        neg_strand = sum(strand1 == "-"), 
        pos_strand = sum(strand1 == "+")
    )

## 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gi |> filter(seqnames1 == 'chr11')

gi |> filter(start1 >= 1e8)

gi |> filter(seqnames1 == seqnames2)

## 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## This only keeps the "type" column from the metadata columns, 
## using <tidy-select> methodology

gi |> select(type)

gi |> select(contains("typ"))

gi |> select(starts_with("ty"))

## 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## This does not restrict to `seqnames1` and `seqnames2` columns. 

gi |> select(starts_with('seq')) 

## 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## This selects `seqnames1` and `seqnames2` columns but converts the output
## into a `DataFrame`.

gi |> select(starts_with('seq'), .drop_ranges = TRUE) 

## 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## This only retains specific pair indices

gi |> slice(1, 2)

gi |> slice(-3)

## 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gr <- GRanges(c("chr1:25-30:-", "chr2:16-20:+"))
gi$id <- seq_len(length(gi))
gr$id <- seq_len(length(gr))

## This returns the `GInteractions` entries overlapping with a `GRanges`
## (with either of both anchors)

find_overlaps(gi, gr)

## This overlap requires the same strandness between 
## the `GInteractions` anchors and the `GRanges` object

find_overlaps_directed(gi, gr)

## This counts how many times each entry in a `GInteractions` object 
## overlaps with a `GRanges` object (with either of both anchors)

count_overlaps(gi, gr)

count_overlaps_directed(gi, gr)

## This filters a `GInteractions` object to only retain the entries 
## overlapping (or not) with a `GRanges` (with either of both anchors)

filter_by_overlaps(gi, gr)

filter_by_non_overlaps(gi, gr)

## This performs a left join between `GInteractions` entries and
## a `GRanges` of interest (with/without considering strandness)

join_overlap_left(gi, gr)

join_overlap_left_directed(gi, gr)

## 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## This returns the `GInteractions` entries for which 
## the "first" anchor overlaps with a `GRanges`

gi |> pin_by("first") |> find_overlaps(gr)

## This returns the `GInteractions` entries for which 
## the "second" anchor overlaps with a `GRanges`

gi |> pin_by("second") |> find_overlaps(gr)

## ----"citation"------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------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## Citation info
citation("plyinteractions")

## ----reproduce3, echo=FALSE-------------------------------------------------------------------------------------------
## Session info
library("sessioninfo")
options(width = 120)
session_info()

## ----vignetteBiblio, results = "asis", echo = FALSE, warning = FALSE, message = FALSE---------------------------------
## Print bibliography
PrintBibliography(bib, .opts = list(hyperlink = "to.doc", style = "html"))